How to pronounce“rnas”in English

small RNAs are expressed in.

messenger RNAs, this the RNAs that encode the proteins within that cell by looking at the small RNA profiles and the messenger RNA profiles that really

strategy for getting small RNAs into cells.

of micr rnas and how they work um so he's tremendous and yeah and it was I was his second person in his lab so he just started at MIT he had a spectacular

translate viral rnas and make more and more and more and more of them that's the first 20 proteins in fact

these negative strand rnas now make positive strand rnas that then look to the human whole cell just like any other

really founded upon our work in small RNAs.

a specific repertoire of small RNAs that it uses to regulate genes.

example the lipid nanoparticles that coat the rnas are Distributing themselves around the body in a way that will have unknown consequences

so you synthesize a thousand different guide rnas you now take each of these guide rnas and you insert them in a pool of cells

positive strand the negative strands sub genomic rnas these can actually recombine and if you have two different viruses infecting the same cell they can actually mix and match between

The basic life is that at any moment messenger RNAs are going from the DNA and the nucleus of our cells

the center and 1 2 3 4 5 6 seven rnas these are like mini chromosomes which

here which is the pb1 which is one of the uh rnas one of the mini chromosomes

And so we started off by asking what spectrum of small RNAs these um devil facial tumors expressed.

does and that sequence can be used to recognize complimentary RNAs um that share sort of the the inverse of that sequence the mirror image of that

is we've looked at the um small RNAs that the cell makes in order to regulate its own genes and compared um the spectrum of those small RNAs in normal

they're able to maintain thirty thousand so sars cov2 rnas thirty thousand bases

is it gonna be made of proteins right or maybe rnas right so we see that you know the the

very interestingly uh I mentioned this pb1 Gene one of these eight rnas uh is

work on um cancer biology usually with focus on the roles of small RNAs in cancer and and ways in which we can use smaller as a tool to understand cancer.

like DNA does and that sequence can be used to recognize complimentary RNAs um

order to regulate its own genes and compared um the spectrum of those small RNAs in normal cells versus tumor cells.

work on um cancer biology usually with focus on the roles of small RNAs in cancer and and ways in which we can use smaller as a tool to understand cancer.

that there were a set of microRNAs which are what these um endogenous um small RNAs are called that differed between the normal cells and the tumor cells.

seemed to it started in a pig but it had bird it had rnas from bird influenza viruses these viruses are all reassortance of

and instead of using a single guide rna you can use a library of guide rnas generated exactly the same way using this array technology

We work on the biology of small RNAs.

So um one is we've looked at the um small RNAs that the cell makes in

components of the RNAi machinery, all the proteins that actually bind to the set of small RNAs and that the smaller program to do these regulatory events

delivery agents are the other um sort of arm of of research into RNAi delivery encapsulating RNAs in lipids in polymers.

We work on the biology of small RNAs.

inside the virus particle and then when the minus rna is in the cell the virus enzyme makes plus rnas and those get translated

and you just sequence small molecules just like you would sequence the genome basically reverse transcribe the small rnas

approach this problem is by taking um all of the components of the RNAi machinery, all the proteins that actually bind to the set of small RNAs

and yet this virus goes in throws in a single messenger rna just like any messenger rna we have tens of thousands of messenger rnas in our

to basically say to the ribosome don't translate this one junk don't look at this one junk and it only spares its own rnas